期刊
BIOCHEMISTRY-MOSCOW
卷 81, 期 3, 页码 187-200出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297916030019
关键词
fusion tags; chromatography; enhancement of protein solubility
资金
- Russian Science Foundation [16-14-00002]
- Russian Science Foundation [16-14-00002] Funding Source: Russian Science Foundation
Virtually all recombinant proteins are now prepared using fusion domains also known as tags. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.
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