期刊
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
卷 44, 期 5, 页码 492-499出版社
WILEY
DOI: 10.1002/bmb.20970
关键词
Streptomyces; nogalamycin; rhodomycin; secondary metabolites
资金
- Finnish Cultural Foundation (Central Foundation)
- Finnish Cultural Foundation (Varsinais-Suomi Regional Funds)
- Alfred Kordelin Foundation
- Vilho, Yrjo ja Kalle Vaisala Foundation
The Streptomyces genetics and secondary metabolism laboratory course gives an introduction to the versatile soil dwelling Gram-positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. (C) 2016 by The International Union of Biochemistry and Molecular Biology.
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