期刊
BIOCHEMISTRY
卷 55, 期 46, 页码 6359-6362出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.6b01003
关键词
-
资金
- National Institutes of Health [R01 CA073808]
- IBS [IBS-R021-D1]
Ribonuclease inhibitor (RNH1) is a cytosolic protein that binds with femtomolar affinity to human ribonuclease 1 (RNase 1) and homologous secretory ribonucleases. RNH1 contains 32 cysteine residues and has been implicated as an antioxidant. Here, we use CRISPR-Cas9 to knock out RNH1 in HeLa cells. We find that cellular RNH1 affords marked protection from the lethal ribonucleolytic activity of RNase 1 but not from oxidants. We conclude that RNH1 protects cytosolic RNA from invading ribonucleases.
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