期刊
ACS INFECTIOUS DISEASES
卷 5, 期 11, 页码 1926-1935出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.9b00273
关键词
antibiotic resistance; efflux pump; Gram-negative bacteria; molecular dynamics simulations; free-energy calculations
资金
- National Institutes of Health [R01-AI052293, R01-GM123169]
- Office of Science of the U.S. Department of Energy [DE-AC05-00OR22725]
- NSF [OCI-1053575, TG-MCB130173]
Multidrug efflux pumps of pathogenic, Gram-negative bacteria comprise an innate resistance mechanism and are key contributors to the emerging global pandemic of antibiotic resistance. Several increasingly detailed cryo-electron microscopy maps have been resolved of an entire efflux pump complex, AcrAB-TolC, resulting in atomistic structural models. Using a recent model, we have carried out nearly 40 mu s of molecular dynamics simulations to study one of the key components of the protein complex AcrA, the membrane fusion protein that connects the inner-membrane-bound AcrB to the outer-membrane-bound TolC. We determined a three-dimensional potential of mean force (PMF) for AcrA, which displays two main conformational basins representing assembly competent and incompetent states. Corresponding experiments show that stabilizing mutations at an interdomain interface shift the dynamic equilibrium between these states to the incompetent one, disrupting pump assembly and function and resensitizing bacteria to existing antibiotics. The modulation of AcrA dynamics through pharmacological intervention therefore presents a promising route for the development of new antibiotics.
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