期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 5, 期 10, 页码 4985-4993出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b00504
关键词
recombinant pyriform silk; silk protein structure and morphology; wet-spinning and automated postspin draw; strong and extensible fibers; tunable mechanical properties
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN/05907-2017]
- NSERC Discovery Accelerator Supplement [RGPAS/507805-2017]
- NSERC CREATE Training Program in BioActives [510963]
- NSERC
- Canadian Foundation for Innovation
- Dalhousie Medical Research Foundation
Pyriform silk is a critically important fiber in spider web construction. It functions in a glue-coated attachment disc to form junctions in spider webs, connecting the web to disparate materials. Despite the biological importance of this silk, both natural and recombinant pyriform silk have yet to be structurally or mechanically characterized. In this study, we demonstrate recombinant production of a 477 amino acid protein based on Argiope argentata pyriform silk. This pyriform silk protein shows alpha-helicity in both an aqueous buffer and in a fluorinated acid- and alcohol-based spinning dope. Wet-spinning produced fibers having no visible defects in surface or cross-sectional analysis, with mechanical behavior varying as a function of postspin stretching conditions and correlating to loss of alpha-helicity in the fibrous state. Multiple conditions gave rise to fibers that are both strong and extensible, contrasting with some other silks that are biased toward being strong or extensible. This behavior is strikingly similar to recombinant aciniform silk, despite distinct primary structuring and composition.
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