期刊
ACS BIOMATERIALS SCIENCE & ENGINEERING
卷 5, 期 10, 页码 5412-5421出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01041
关键词
ACL reconstruction; ACL regeneration; knitted silk-collagen sponge scaffold; ligament stem/progenitor cells; cell sheet; osteoarthritis prevention
资金
- National Key R&D program of 573 China [2017YFA0104900, 2017YFA0104901]
- National High Technology Research and Development Program of China (863 Program) [2012AA020503]
- NSFC [01103603, 31271041, 81125014, 81201396, 81271970, 81201395, 81330041, 81572157, 81572115]
- National Key Scientific Program [2012CB966604]
- Regenerative Medicine in Innovative Medical Subjects of Zhejiang Province
- General Scientific Research Project of Zhejiang Provincial Education Department [Y201738521]
- Medical Science and Technology Project of Zhejiang Province [201341741, 2018KY818]
- Zhejiang Province [Z2100086, LY12H06006, LR14H060001, LY14H060003]
- Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents
Current surgical management of anterior cruciate ligament (ACL) rupture still remains an intractable challenge in ACL regeneration due to the weak self-healing capability of ACL. Inadequate cell numbers and vascularization within the articular cavity contribute mainly to the poor prognosis. This time, we fabricated a new tissue engineering scaffold by adding ligament stem/progenitor cell (LSPC) sheets to our previous knitted silk-collagen sponge scaffold, which overcame these limitations by providing sufficient numbers of seed cells and a natural extracellular matrix to facilitate regeneration. LSPCs display excellent proliferation and multilineage differentiation capacity. Upon ectopic implantation, the knitted silk-collagen sponge scaffold incorporated with an LSPC sheet exhibited less immune cells but more fibroblast-like cells, deposited ECM and neovascularization, and better tissue ingrowth.In a rabbit model, we excised the ACL and performed a reconstructive surgery with our scaffold. Increased expression of ligament-specific genes and better collagen fibril formation could be observed after orthotopic transplantation. After 6 months, the LSPC sheet group showed better results on ligament regeneration and ligament-bone healing. Furthermore, no obvious cartilage and meniscus degeneration were observed at 6 months postoperation. In conclusion, these results indicated that the new tissue engineering scaffold can promote ACL regeneration and slow down the progression of osteoarthritis, thus suggesting its high clinical potential as an ideal graft in ACL reconstruction.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据