期刊
POLAR SCIENCE
卷 22, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.polar.2019.08.003
关键词
Antarctic lichen extract; Antibacterial; Antioxidant; Cytotoxic activity; Usnea antarctica; Usnea aurantiaco-atra
The lichens are symbiotic organisms, synthesize a great variety of chemically complex. Natural and potential source of bioactive compounds. U. antarctica and U. aurantiaco-atra were collected during 23rd Peruvian Scientific Expedition (ANTAR XXIII-2015) from the Antarctic Scientific Station Machu Picchu and were transferred to the ITP for processing. The samples were dried and grounded, after that, an extraction with acetone (1:10 w/v) also with methanol (1:10 w/v) was performed. Both extracts were mixed and vacuumed dried (30 degrees C). A methanol-acetone extract (MAE) from each lichen was obtained. MAE from U. antarctica showed a major concentration of total phenols (22.80 +/- 0.08 mg GA/g MAE) than U. aurantiaco-atra (19.42 +/- 0.32 mg GA/g MAE). Besides, U. antarctica exhibited a superior value of inhibition of ABTS(center dot+) radical (89.05 +/- 0.01 mu mol TE/g MAE) than U. aurantiaco-atra (79.84 +/- 0.09 mu mol TE/g MAE). The antibacterial activity of MAEs against Staphylococcus aureus ATCC 14775, Pseudomonas aeruginosa ATCC 27853 and Vibrio alginolyticus ATCC 17749 was performed however was only demonstrated against S. aureus. The minimum inhibitory concentration (MIC) was evaluated, U. antarctica and U. aurantiaco-atra exhibited 94.76% and 98.43%, respectively of inhibition bacterial growth at 31.25 mu g/mL of MIC value. MAE of U. antarctica (IC50 = 169.64 mu g/mL) and U. aurantiaco-atra (IC50 = 270.82 mu g/mL).
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