期刊
PEERJ
卷 7, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.7744
关键词
Microbiome; Microbial ecology; Morchella; USEARCH; Mushroom cultivation; CONSTAX; Amplicon sequencing; Pedobacter
资金
- Michigan State University AgBioResearch NIFA project [MICL02416, GREEEN GR17-083]
- Science and Technology Service Network Initiative, Chinese Academy of Sciences
- Guizhou Science and Technology Program (2018) [4002]
- Plant Biotechnology for Health and Sustainability Training Program [NIH T32-GM110523]
Morels (Morchella spp.) are iconic edible mushrooms with a long history of human consumption. Some microbial taxa are hypothesized to be important in triggering the formation of morel primordia and development of fruiting bodies, thus, there is interest in the microbial ecology of these fungi. To identify and compare fungal and prokaryotic communities in soils where Morchella sextelata is cultivated in outdoor greenhouses, ITS and 16S rDNA high throughput amplicon sequencing and microbiome analyses were performed. Pedobacter, Pseudomonas, Stenotrophomonas, and Flavobacterium were found to comprise the core microbiome of M. sextelata ascocarps. These bacterial taxa were also abundant in the soil beneath growing fruiting bodies. A total of 29 bacterial taxa were found to be statistically associated to Morchella fruiting bodies. Bacterial community network analysis revealed high modularity with some 16S rDNA operational taxonomic unit clusters living in specialized fungal niches (e.g., pileus, stipe). Other fungi dominating the soil mycobiome beneath morels included Morchella, Phialophora, and Mortierella. This research informs understanding of microbial indicators and potential facilitators of Morchella ecology and fruiting body production.
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