期刊
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
卷 65, 期 -, 页码 234-237出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2016.02.009
关键词
Coastal elasmobranch; Microsatellite; Conservation; Habitat degradation; Population genetics; PCR primers
资金
- University of Auckland CRP&M contestable fund [NZGL00225]
Twelve microsatellite loci were developed for the short-tailed stingray Dasyatis brevicaudata using a next-generation sequencing approach, and their utility for population genetics studies was assessed. Eleven out of twelve loci were polymorphic (4-15 alleles) and amplified reliably, with observed heterozygosities between 0.32 and 0.86 (mean = 0.56). Successful cross-species amplification of many of these markers was achieved across seven other dasyatid species currently listed as Data Deficient by the IUCN, suggesting these microsatellites will be a useful resource for the investigation of ecological and conservation genetics in a range of species of conservation concern. (C) 2016 Elsevier Ltd. All rights reserved.
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