期刊
SUGAR TECH
卷 22, 期 1, 页码 52-64出版社
SPRINGER INDIA
DOI: 10.1007/s12355-019-00755-4
关键词
Intercropping; Microbial community; Next-generation sequencing; NifH gene; Sugarcane
类别
资金
- GXAAS [GNKB2014021]
- National High Technology Research and Development Program (``863'' Program) of China [2013AA102604]
- National Natural Science Foundation of China [31171504, 31101122, 31471449]
- Guangxi Special Funds for Bagui Scholars's and Distinguished Experts
- Guangxi Natural Science Foundation
- Guangxi Academy of Agriculture Sciences Fund [2011GXNSFF018002, 2012GXNSFDA053011, 2013NXNSFAA019073, GuiNongKe2014YD01]
The present study discussed the application of the intercropping system to improve land use efficacy and soil microbial activity. We assessed linkages of soil properties and unculturable diazotrophs community under three cultivation systems (monoculture sugarcane, peanut-sugarcane and soybean-sugarcane intercropping). Rhizosphere soil of sugarcane was sampled and DNA was extracted. We amplified the nifH gene and sequenced by high throughput sequencing. The bioinformatics analysis of sequenced data obtained a total of 436,458 nifH gene reads that are classified into 3201 unique operational taxonomic units (OTUs). A higher percentage of exclusive OTUs identified under soybean-sugarcane intercropping (< 375). The microbial structure results showed that Alpha-proteobacteria and Beta-proteobacteria were the dominant groups in all three cultivation systems. While genus such as Bradyrhizobium, Burkholderia, Pelomonas, and Sphingomonas was predominant in the intercropping systems and these diazotrophic bacterial communities were positively correlated to the soil pH and soil enzyme protease. Additionally, a lower quantity of available P in the soil of intercrops indicated a strong link between soil nutrients uptake and microbial activity. The results of the present study concluded some interesting facts of intercropping systems that positively improved the soil microbial activity and this kind of strategy could help to cultivate multiple crops to improve the economic growth of the country by sustainable sugarcane production.
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