期刊
MICROMACHINES
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/mi10080537
关键词
Lab-on-a-chip device; POC; high-risk HPV genotypes; molecular diagnosis
类别
资金
- Key Research Program of the Chinese Academy of Sciences [KGFZD-135-18-019]
- Program of the Science and Technology Service Network Initiative
- Key Research and Development Program of Anhui Province of China [1804c04020335/1804a09020051]
- Youth Innovation Promotion Association, Chinese Academy of Sciences [2018489]
- Dean's Fund of Hefei Institute of Physical Science, China
Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.
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