期刊
MICROMACHINES
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/mi10080527
关键词
polyethylene glycol; hydrogel microbead; microfluidic channel; on-bead bioassay; peptide synthesis
类别
资金
- Korea and Basic Science Research Program through the National Research Foundation of Korea [NRF-2018R1D1A1A09083883, NRF-2019R1F1A1062208]
Microfluidic generation of hydrogel microbeads is a highly efficient and reproducible approach to create various functional hydrogel beads. Here, we report a method to prepare crosslinked amino-functionalized polyethylene glycol (PEG) microbeads using a microfluidic channel. The microbeads generated from a microfluidic device were evaluated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and confocal laser scanning microscopy, respectively. We found that the microbeads were monodisperse and the amino groups were localized on the shell region of the microbeads. A swelling test exhibited compatibility with various solvents. A cell binding assay was successfully performed with RGD peptide-coupled amino-functionalized hydrogel microbeads. This strategy will enable the large production of the various functional microbeads, which can be used for solid phase peptide synthesis and on-bead bioassays.
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