期刊
MICROMACHINES
卷 10, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/mi10090624
关键词
polymer; microfluidic chips; point of care; three-dimensional microstructures; protocol
类别
资金
- European Union [773422, 687697]
- H2020 Societal Challenges Programme [773422] Funding Source: H2020 Societal Challenges Programme
This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).
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