期刊
BIOCHEMICAL JOURNAL
卷 473, 期 -, 页码 1443-1453出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20151366
关键词
Bacillus megaterium; biotransformation; mesotrione herbicide; nitroreductase enzyme
资金
- Agence Nationale de la Recherche [ANR-13-CESA-0002 TRICETOX]
- Region Auvergne
- French Ministry for Higher Education and Research
- European Regional Development Fund
- French Infrastructure for Integrated Structural Biology [FRISBI ANR-10-INSB-05-01]
Mesotrione is a selective herbicide belonging to the triketone family, commonly used on maize cultures since 2003. A mesotrione-transforming Bacillus megaterium Mes11 strain isolated from an agricultural soil was used as a model to identify the key enzymes initiating the biotransformation of this herbicide. Two enzymes (called NfrA1 and NfrA2/YcnD) were identified, and functionally and structurally characterized. Both belong to the NfsA FRP family of the nitro-FMN reductase superfamily (type I oxygen-insensitive nitroreductase) and show optimal pH and temperature of 6-6.5 and 23-25 degrees C, respectively. Both undergo a Ping Pong Bi Bi mechanism, with NADPH and NADPH/NADH as cofactors for NfrA1 and NfrA2/YcnD, respectively. It is interesting that both can also reduce various nitro compounds including pesticides, antibiotics, one prodrug and 4-methylsulfonyl-2-nitrobenzoic acid, one of the mesotrione metabolites retrieved from the environment. The present study constitutes the first identification of mesotrione-transforming enzymes. These enzymes (or their corresponding genes) could be used as biomarkers to predict the capacity of ecosystems to transform mesotrione and assess their contamination by both the parent molecule and/or the metabolites.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据