期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 42, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.algal.2019.101603
关键词
-
资金
- Nuno Nunes [M1420-01-0247-FEDER000002, MAC/1.1b/086]
- Agencia Regional para o Desenvolvimento da Investigacao Tecnologia e Inovacao (ARDITI) [M1420-09-5369-FSE-000001]
- DRCT (Azores Regional Government)
- Portuguese National Funds, through FCT - Fundacao para a Ciencia e a Tecnologia - FEDER
- [UID/BIA/00329/2013]
- Fundação para a Ciência e a Tecnologia [UID/BIA/00329/2013] Funding Source: FCT
Fucoxanthin is considered an important marine bioactive compound with biological properties with promising effects, namely on health. A simple and efficient analytical methodology is proposed for its quantification in seaweed biomass by using vortex-assisted solid-liquid microextraction (VASLME) followed by reversed phase high-performance liquid chromatography (RP-HPLC) photodiode array detection (PDA) analysis. This microextraction uses reduced quantities of sample (25 mg) and solvent (300 mu L of ethanol) to efficiently extract this high-valued xanthophyll, in a vortex time of 15 min. These extraction parameters were optimized performing a Central Composite Design (CCD) analysis, running 32 individual experiments. In turn, the method validation was assessed. The linearity of the method was confirmed (R-2=0.99998) in a concentration range from 12 to 3600 mu g.g(-1) dw. Also, good sensitivity and accuracy results were observed through the LOD (3.33 mu g.g(-1)), LOQ (10.09 mu g.g(-1)) and recovery (varied from 95 to 97%) assessments. Good precision was also verified, with intra-day variation within 2.0-3.3%, and inter-day within 1.0-3.8%. Matrix effect was also evaluated and an acceptable variation of 3.4% was found. The method applicability was confirmed by the analysis of 22 seaweed biomass samples and fucoxanthin content was found to vary from about 10 to 853 mu g.g(-1) dw. This method demonstrated a good performance and can be successfully implemented for a rapid, reliable and accurate screening of fucoxanthin in seaweed biomass.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据