期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 112, 期 -, 页码 241-248出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2016.04.030
关键词
Phenol; Biogas; Microbial community analysis; Waste-Water treatment; Bioreactors; Anaerobic processes
资金
- Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) from the Ministry of Trade, Industry & Energy, Republic of Korea [20132020000170]
- National Research Foundation of Korea (NRF) grant - Korean Government (MSIP: Ministry of Science, ICT and Future Planning) [NRF-2015R1C1A1A02037289]
The objective of this study was to investigate microbial communities in a continuous anaerobic phenol degrading system using a next generation sequencing tool. The anaerobic granules adapted to phenol were first obtained by repeated-batch operation, which were then inoculated in an up-flow anaerobic sludge blanket reactor (UASB) operated at various organic loading rates (OLRs). Lag periods for both phenol degradation and CH4 production decreased as batch fermentation was repeated, indicating a progressive adaptation of the granules to phenol. In the UASB operation, the highest OLR handled was 6 kg COD/m(3)/d, in which the attained biogas production rate, phenol degradation, and CH4 contents were 2.1 m(3)/m(3)/d, 79.0%, and 75.3%, respectively. Syntrophorhabdus and Clostridium were found to be the dominant bacteria, whose sum occupied around 60% of total bacterial sequences. In particular, there was a significant increase in Syntrophorhabdus (39.2% of total bacterial sequences), known to degrade phenol to benzoate and subsequently to acetate and hydrogen in syntrophic association with a hydrogenotrophic methanogen. In terms of archaea, Methanosaeta (42.1% of total archaeal sequences), and Methanobacterium (24.5% of total archaeal sequences) became dominant as operation continued, which were negligible in the inoculum. (c) 2016 Elsevier B.V. All rights reserved.
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