期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 105, 期 -, 页码 90-96出版社
ELSEVIER
DOI: 10.1016/j.bej.2015.09.013
关键词
Biosynthesis; Butanol tolerance; Atmospheric and room-temperature plasmas; Fed-batch culture; Pervaporation; Process integration
资金
- 973 Program of China [2011CB707405]
- National Natural Science Foundation of China [21106067]
- 863 Program of China [2011AA02A208]
- State Key Laboratory of Materials-Oriented Chemical Engineering Foundation of Nanjing University of Technology [ZK201001]
- Program for New Century Excellent Talents in University
- Program for Changjiang Scholars and Innovative Research Team in University [06-A-047]
- Jiangsu Province Natural Science Foundation [BK20140940]
Butanol inhibition is one of the major obstacles limiting the economic viability of acetone-butanol-ethanol (ABE) fermentation. In this study, a butanol-tolerant mutant (Clostridium beijerinckii BT14) was generated by atmospheric and room temperature plasmas (ARTP). This mutant showed significant advantage over its parent strain in terms of butanol tolerance. Compared to its parent strain, batch fermentation by this mutant produced 25% higher butanol and 33% higher ABE solvents due to its efficient generation of intracellular NADH and high NADH-dependent butanol dehydrogenase activity. Furthermore, C. beijerinckii BT14 was applied to fed-batch fermentation with pervaporation (PV). As a results, C. beijerinckii BT14 grew to a high cell density and this process generated highly concentrated ABE solution with a high solvent productivity of 0.98 g/(L h) and glucose consumption rate of 2.64 g/(L h). Thus, this work provides an appropriate strategy to develop an efficient process for ABE production in the PV coupled fermentation. (C) 2015 Elsevier B.V. All rights reserved.
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