期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 105, 期 -, 页码 159-167出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2015.09.016
关键词
Glucose; Microbial growth; Fermentation; Fed-batch culture; 150l bioreactor; 2,3-Butanediol (2,3-BD)
资金
- Department of Biotechnology, India [BT/HRD/35/01/02/2009]
This study was focused on scaling up of 2,3-butanediol (2,3-BD) production, via fermentation in a 1501 bioreactor using the newly isolated strain Enterobacter cloacae TERI BD 18. Operational parameters were customized for the strain E. cloacae using a fed- batch strategy, which enhanced the glucose to 2,3-BD conversion yield (0.44 g/g). Furthermore, dual stage pH and agitation control regime was considered where in the initial 10 h the pH value was 7.5 and the agitation rate used was 200 rpm favoring bacterial growth, while these values were subsequently changed to a pH value of 6.5 and an agitation rate of 150 rpm leading to enhanced accumulation of 2,3-BD. Production of 85 g/l of 2,3-BD was achieved with ethanol and acetoin as the only by-products. Total productivity of 1.73 g/l/h with a yield of 0.48 g of 2,3-BD per g of glucose was achieved in the 1501 bioreactor. Additionally, the total fermentation time was reduced to 50 h with this strategy. The integrated approach of fed-batch strategy together with dual regime in pH and agitation control favors 2,3-BD production in the pilot scale bioreactor system employed in this study. (C) 2015 Elsevier B.V. All rights reserved.
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