期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 113, 期 -, 页码 141-151出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2016.06.006
关键词
Antioxidant; Ginseng by-product; Pressurized hot water extraction; Total saponins; Total phenolic content
资金
- Business for Cooperative R&D between Industry, Academy and Research Institute - Korea Small and Medium Business Administration [C0213260]
- Korea Evaluation Institute of Industrial Technology (KEIT) [C0213260] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study investigates the influence of process conditions on pressurized hot water extraction (PHWE) to maximize the bioactive compounds, including total saponins (TS), total phenolic compounds (TP) and total antioxidants (TA), produced from ginseng by-product (GBP). Response surface methodology (RSM) was employed to study the influences of temperature [180-24 degrees C], pressure [15-45 bar], solid to liquid (S/L) ratio [0.04-0.09 g/mL], agitation speed [100-200 RPM], and reaction time [5-15 min]. Box-Behnken design (BBD) was used with five variables and three levels. The optimal conditions were 207 degrees C, 43.45 bar, S/L ratio of 0.04 g/mL, agitation speed of 199 RPM, and reaction time of 15 min. The obtained values of TS, TP and TA under these conditions were: 7.12 g Gin Re/100 g, 49.11 mg GAE/g, and 5.31 g TE/100 g, respectively. The optimized ginseng hydrolysate showed good antimicrobial and antihypertensive activities. Homogentisic and gentisic acid were the main phenolic compounds present in the ginseng hydrolysate. (C) 2016 Elsevier B.V. All rights reserved.
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