Korean Red Ginseng aqueous extract improves markers of mucociliary clearance by stimulating chloride secretion

Background: Abnormal chloride (Cl-) transport has a detrimental impact on mucociliary clearance in both cystic fibrosis (CF) and non-CF chronic rhinosinusitis. Ginseng is a medicinal plant noted to have anti-inflammatory and antimicrobial properties. The present study aims to assess the capability of red ginseng aqueous extract (RGAE) to promote transepithelial Cl- secretion in nasal epithelium. Methods: Primary murine nasal septal epithelial (MNSE) [wild-type (WT) and transgenic CFTR-/-], fisher-rat-thyroid (FRT) cells expressing human WT CFTR, and TMEM16A-expressing human embryonic kidney cultures were utilized for the present experiments. Ciliary beat frequency (CBF) and airway surface liquid (ASL) depth measurements were performed using microeoptical coherence tomography (mu OCT). Mechanisms underlying transepithelial Cl- transport were determined using pharmacologic manipulation in Ussing chambers and whole-cell patch clamp analysis. Results: RGAE (at 30 mu g/mL of ginsenosides) significantly increased Cl- transport [measured as change in short-circuit current (Delta I-SC = mA/cm(2))] when compared with control in WT and CFTR-/- MNSE (WT vs control = 49.8-2.6 vs 0.1+/-0.2, CFTR-/- = 33.5-1.5 vs 0.2-0.3, p < 0.0001). In FRT cells, the CFTRmediated DISC attributed to RGAE was small (6.8 - 2.5 vs control, 0.03 - 0.01, p < 0.05). In patch clamp, TMEM16A-mediated currents were markedly improved with co-administration of RGAE and uridine 5-triphosphate (8406.3 +/- 807.7 pA) over uridine 5-triphosphate (3524.1 +/- 292.4 pA) or RGAE alone (465.2 +/- 90.7 pA) (p < 0.0001). ASL and CBF were significantly greater with RGAE (6.2+/-0.3 mm vs control, 3.9+/-0.09 mm; 10.4+/-0.3 Hz vs control, 7.3 - 0.2 Hz; p < 0.0001) in MNSE. Conclusion: RGAE augments ASL depth and CBF by stimulating Cl- secretion through CaCC, which suggests therapeutic potential in both CF and non-CF chronic rhinosinusitis. (C) 2019 The Korean Society of Ginseng, Published by Elsevier Korea LLC.

Automated summary

The study revealed that red ginseng aqueous extract can enhance airway surface liquid depth and ciliary beat frequency by promoting chloride secretion in nasal epithelium, indicating its therapeutic potential in both CF and non-CF chronic rhinosinusitis.