期刊
JOURNAL OF FUNCTIONAL FOODS
卷 59, 期 -, 页码 329-336出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2019.06.007
关键词
Ribes spp.; Intestinal inflammation; COX-2; iNOS; Interleukin secretion
资金
- Ramon y Cajal program [2012_11910]
- Ministry of Economy and Competitiveness, Spain [AGL2016-77288-R]
- CONICYT-PCHA/Doctorado Nacional [2015-21151561]
- PIEI-QUIM-BIO, Universidad de Talca
The polyphenol-enriched extracts (PEEs) of Chilean currants Ribes magellanicum and R. punctatum were submitted to in vitro gastro-intestinal digestion and their anti-inflammatory activities were assessed using differentiated human Caco-2 (clone C2BBe1) cells stimulated with interleukin 1 beta (IL-1 beta). The inhibitory effect of non digested and digested PEEs towards human cyclooxygenase 1 (COX-1) and COX-2, and the gene expression of COX-2 and inducible nitric oxide synthase (iNOS) was also evaluated. The digested PEE from R. punctatum decreased the secretion of IL-8, IL-6, and TNF-alpha; whereas R. magellanicum reduced IL-6 and TNF-a in the Caco-2 cells (p < 0.05). Both digested extracts significantly down-regulated the mRNA expression of COX-2 and iNOS (p < 0.05). PEEs showed 60% of inhibition towards COXs, with higher inhibition against COX-2. The PEEs from R. punctatum displayed better anti-inflammatory activity in all the experiments. Our results suggest that R. magellanicum and R. punctatum might be useful against intestine inflammatory conditions.
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