期刊
VIRUSES-BASEL
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/v11080761
关键词
morbillivirus; hemagglutinin; SLAM; canine distemper virus; measles virus; surface plasmon resonance; structure
类别
资金
- Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [18am0101093j0002]
- Hokkaido University
- Global Facility Center (GFC)
- Pharma Science Open Unit (PSOU) - MEXT
- Hokkaido University Biosurface project
- Takeda Science Foundation
- JSPS KAKENHI [23770102, 25870019, JP17H05827]
- JSPS Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation
- AMED J-PRIDE [JP18fm0208022h]
- Naito Foundation Subsidy for Female Researchers after Maternity Leave
- Support Office for Female Researchers at Hokkaido University
- Grants-in-Aid for Scientific Research [23770102, 25870019] Funding Source: KAKEN
Measles virus (MV) and canine distemper virus (CDV) are highly contagious and deadly, forming part of the morbillivirus genus. The receptor recognition by morbillivirus hemagglutinin (H) is important for determining tissue tropism and host range. Recent reports largely urge caution as regards to the potential expansion of host specificities of morbilliviruses. Nonetheless, the receptor-binding potential in different species of morbillivirus H proteins is largely unknown. Herein, we show that the CDV-H protein binds to the dog signaling lymphocyte activation molecule (SLAM), but not to the human, tamarin, or mouse SLAM. In contrast, MV-H can bind to human, tamarin and dog SLAM, but not to that of mice. Notably, MV binding to dog SLAM showed a lower affinity and faster kinetics than that of human SLAM, and MV exhibits a similar entry activity in dog SLAM- and human SLAM-expressing Vero cells. The mutagenesis study using a fusion assay, based on the MV-H-SLAM complex structure, revealed differences in tolerance for the receptor specificity between MV-H and CDV-H. These results provide insights into H-SLAM specificity related to potential host expansion.
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