期刊
VIRUS RESEARCH
卷 270, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.virusres.2019.197662
关键词
Human adenovirus type 4; DsRed; Vector; Neutralizing antibody
类别
资金
- National Natural Science Foundation of China [NSFC 31570163]
- National Key Research and Development Program of China [2018YFC1200100]
- Youth Project of State Key Laboratory of Respiratory Disease [SKLRD-QN-201713]
- National Science and Technology Major Project for Innovative Pharmaceuticals of China [2015ZX09J15105-002]
- Science and Technology Key Project of Guangzhou [201803040004, 201504010032]
Human adenovirus type 4 (HAdV4) is an etiological agent of acute respiratory disease (ARD) in pediatric and adult patients. HAdV4 strains can be divided into two major genomic clusters, namely prototype (p)-like viruses and a-like viruses. Here, the complete genome sequence of HAdV4 strain GZ01, isolated from a child with ARD in southern China, is first reported and analyzed. This strain was determined to be of the 4a1 genome-type based on in silica restriction profiles. Then, a replication-competent rAd4DsRed virus, containing the HAdV4 GZ01 infectious genome and expressing the reporter molecule DsRed, was generated and characterized. Recombinant rAd4DsRed can infect AD293, hamster, and mouse cells in which DsRed protein was expressed. No changes in antigenicity and genome replication were detected for rAd4DsRed and wild-type HAdV4. Mice immunized with rAd4DsRed was elicited a marked antibody response to DsRed. A rapid method of testing neutralizing antibodies against HAdV3 and HAdV4 was also established using a mixture of rAd4DsRed and rAd3EGFP. Our results provide the foundation to develop HAdV4 vaccines, potential vector platforms for vaccine and gene therapy, and rapid methods for serological and antiviral screening.
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