期刊
TOXICOLOGY IN VITRO
卷 58, 期 -, 页码 224-229出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2019.03.041
关键词
PFDA; Oocyte maturation; Intracellular calcium; GJIC; Pig
类别
资金
- CONACYT [0105961-M]
- SEP-CONACYT [180043]
- [CONACYT-PNPC003797]
- [CONACYT-CVU508753]
- [509499]
Perfluorodecanoic acid (PFDA) is a synthetic perfluorinated compound, which has been reported to exert adverse effects on somatic cells. However, its effects on germ cells have not been studied to date. The aim of the present study was to analyze the effects of PFDA on the viability, intracellular calcium levels and gap junction intercellular communication (GJIC) during porcine oocyte maturation in vitro. PFDA negatively impacted oocyte viability (medium lethal concentration, LC50 = 7.8 mu M) and maturation (medium inhibition of maturation, IM50 = 3.8 mu M). Oocytes exposed to 3.8 mu M PFDA showed higher levels of intracellular calcium relative to control oocytes. In addition, GJIC among the cumulus cells and the oocyte was disrupted. The effects of PFDA on oocyte calcium homeostasis and intercellular communication seem to be responsible for the inhibition of oocyte maturation and oocyte death. In addition, since the deleterious effects of PFDA on oocyte viability, maturation and GJIC are significantly stronger than the previously reported effects of another widely used perfluorinated compound (Perfluorooctane sulfonate) in the same model, the use of PFDA in consumer products is questioned.
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