期刊
TOXICOLOGY AND APPLIED PHARMACOLOGY
卷 382, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2019.114734
关键词
Gefitinib; Curcumin; gamma-PGA-Cur/Gef nanoparticles; SAS human oral cancer cells; Apoptosis
资金
- Ministry of Science and Technology, Taipei, Taiwany [MOST 106-2320-B-039 -056 -]
- China Medical University [CMU-103-S-26]
Curcumin (Cur), a natural product, has been shown to have anti-tumor activities in many human cancer cells. Gefitinib (Gef) is a clinical drug for cancer patients. However, there is no available information to show whether Gef/Cur nanoparticles (NPs) increased cell apoptosis and anti-tumor effects on xenograft mice model in vivo. In this study, gamma-polyglutamic acid-coated nanoparticles loaded with Gef and Cur (gamma-PGA-Gef/Cur NPs) were developed and its physicochemical properties and antitumor effects were investigated in vitro and in vivo. The gamma-PGA-Gef/Cur NPs showed 548.5 +/- 93.7 nm in diameter and -40.3 +/- 3.87 mV on surface charge. The loading efficiencies of Gef and Cur were 89.5 and 100%, respectively. gamma-PGA-Gef/Cur NPs could be internalized into SAS cells and significantly decreased total cell viability of SAS cells. Western blotting results indicated that both free Gef/Cur and gamma-PGA-Gef/Cur NPs induced apoptotic cell death via caspase- and mitochondria-dependent pathways. In vivo studies indicated that treatments of PLGA NPs, free Gef/Cur, and gamma-PGA-Gef/Cur NPs did not significantly affect appearances and bodyweights of mice. But the gamma-PGA-Gef/Cur NPs significantly suppressed tumor size when comparing to free Gef/Cur-treated group. The nanoparticles developed in this study may be used as a potential therapy for oral cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据