期刊
TALANTA
卷 200, 期 -, 页码 57-66出版社
ELSEVIER
DOI: 10.1016/j.talanta.2019.03.007
关键词
Aptamers; Carbon fiber; Graphene; Lysozyme; Chemiluminescence biosensor
资金
- National Science Foundation for Young Scientists of China [21605094]
- Shandong Province Natural Science Institute Joint Fund [ZR2015YL006]
- Natural Science Foundation of Shandong Province, China [ZR2016BM01]
- Horizontal Scientific Research Project of China [W15077]
In our work, aptamers and hemin/G-quadruplex DNAzyme modified sandwich -rod graphene quantum dots @ graphene oxide @ carbon fiber composite (DNAzyme/L-Apt/GQDs@GO@CF) was successfully prepared for sensitive and selective chemiluminescence (CL) detection of lysozyme (LZM). Initially, GQDs@GO@CF was successfully prepared and characterized. Lysozyme aptamers (L-Apt) as a recognition element and hemin/Gquadruplex DNAzyme (DNAzyme) as a catalyst of luminal - H2O2 were modified on the surface of GQDs@GO@CF, sequentially. The immobilization properties of GQDs@GO@CF to L-Apt and the adsorption properties of L-Apt/GQDs@GO@CF to DNAzyme were also researched, respectively. Then, the modified sandwich-rod carbon fiber composite was applied to the construction of CL biosensor for LZM detection. When LZM existed, DNAzyme would be released from the surface of L-Apt/GQDs@GO@CF and catalyzed the reaction of luminal - H2O2. Under optimized conditions, the CL biosensor for LZM detection showed wide linear range of 2.64 x 10(-10) to 6.6 x 10(-8) g/L and low detection limit of 1.25 x 10(-11) g/L (3 delta). Finally, the CL biosensor was successfully used for LZM detection in human urine samples and illustrated the potential application in pratical samples.
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