期刊
TALANTA
卷 200, 期 -, 页码 169-176出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2019.03.035
关键词
CTCs; Microvalves; High-purity isolation; On-chip analysis
资金
- National Key Research and Development Program of China [2018YFA0108202, 2017YFA0205300]
- National Natural Science Foundation of China [61571429, 61801464, 61801465]
- STS Project of the Chinese Academy of Sciences [KFJ-STS-SCYD-120]
- Science and Technology Commission of Shanghai Municipality [16410711800, 14391901900, 17511104402]
Circulating tumor cells (CTCs) are rare cancer cells that are shed from the tumors into the peripheral blood and are instrumental in distant metastasis. Early detection of CTCs can therefore improve prognoses and help design patient-specific treatment regimen. However, the current CTC isolation techniques have poor efficacy and selectivity, owing to the rarity and heterogeneity of the CTCs. We designed a microchip for integrated single-cell isolation of CTCs - based on cell size and immuno-phenotype - and analysis. Each isolation unit consisted of a trap channel, a bypass channel, and a release channel. The larger cells were preferentially captured at the trap channels and flushed out selectively via release microvalves according to their immuno-phenotype. The average recovery rate and purity of lung cancer cells isolated from a spiked WBC population were respectively 92.5% and 94% using the microchip, which were significantly higher compared to that obtained using anti-CD45 magnetic beads. In addition, the isolated cancer cells were analyzed on chip for the surface markers of epithelial mesenchymal transition. Taken together, the integrated microchip is a promising tool for the isolation and analysis of CTCs in the clinical setting.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据