期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 293, 期 -, 页码 247-255出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.04.120
关键词
Cysteine; Dual-emission; Two-photon fluorescent probe; Lysosome; Zebrafish
资金
- National Science Foundation of China [21778001]
- Natural Science Foundation of Anhui Province [1608085MB39]
Cysteine (Cys) can effectively maintain lysosomes under a highly reductive environment. However, fluorescent probes for specific detection of lysosomal Cys over other biothiols are rare. Probe Lyso-DCHO utilizing a carbazole-based two-dimensional intramolecular charge transfer (ICT) system was designed to detect Cys with a ratiometric signal (119 nm emission shift). The sensing mechanism was fully supported by H-1-NMR titration, ESI-MS and DFT calculations. Lyso-DCHO can be well localized in lysosomes along with good biocompatibility. Furthermore, Lyso-DCHO achieved a dual-channel fluorescence response toward Cys in lysosomes and zebrafishes with high selectivity under the two-photon (TP) excitation.
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