期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 292, 期 -, 页码 164-170出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.04.128
关键词
Core-shell nanocrystals; Dendritic nanostructures; Carbohydrate antigen 15-3; Ferrocene-grafted chitosan; Immunosensor
资金
- National Natural Science Foundation of China [21475118, 21305124, 21675093]
- Zhejiang Public Welfare Technology Application Research Project [LGG18E010001, LGG19B050001]
- Natural Science Foundation of Zhejiang Province [LQ12B05003]
- Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology [SATM201703, STAM201804]
The introduction of advanced nanocrystals and mediator is crucial for signal amplification of electrochemical immunosensors due to trace amounts of analytes in real samples. Herein, a novel ultrasensitive label-free electrochemical immunosensor was constructed to monitor carbohydrate antigen 15-3 (CA15-3) based on dendritic Au@Pt core-shell nanocrystals (Au@Pt NCs) uniformly dispersed with ferrocene-grafted-chitosan (Fc-g-CS), where Au@Pt NCs were prepared with hexadecyldimethyl benzyl ammonium chloride (HDBAC) as the growth-directing agent via a simple one-pot wet-chemical strategy. More notably, Fc grafted with CS would dramatically improve the stability and the Fc-g-CS enlarged specific surface area and behaved as dispersing agent for the Au@Pt NCs to greatly boost the electrochemical signals and improve the biocompatibility. The resulting immunosensor displayed wider linear range of 0.5-200 U mL(-1) and lower detection limit of 0. 17 U mL(-1), which was further explored for detecting CA15-3 in serum sample with highly acceptable results.
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