期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 294, 期 -, 页码 69-77出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.05.022
关键词
AIE; ESIPT; Cell imaging; Multi-target detection; Cys
资金
- Natural Science Foundation of China [21765014, 21864018, 21365014]
- Nanchang University Graduate Innovation Specialized Fund Project [CX2017042]
In this work, an effective and simple aggregation-induced emission (AIE) active fluorescent probe based on ESIPT luminescence principle, 2-hydroxy-1-naphthal-4-aminoantipyrine (1), was synthesized via a simple chemical reaction. It displayed strong fluorescence enhancement in water. The AIE mechanism of 1 was explored by electron microscopy and computational analysis. We demonstrate the combination effect of intramolecular hydrogen bonds and pi-pi stacking to endow 1 with such a property. Importantly, the ensemble of 1 with copper ions (Cu-1) as a fluorescent probe enables highly selective and sensitive turn-on fluorescence detection of cysteine (Cys) over other interfering substances. Under optimal detection conditions, this probe has a good linear relationship in the concentration range of Cys between 0 and 8 mu M, and the detection limit of Cys was estimated as 84 nM. Furthermore, this assay exhibits good reversibility of 1 to Cu2+ and Cys. Due to its good biocompatibility evaluated by CCK-assay, the probe Cu-1 was finally applied in imaging of Cys within Min6 cells successfully.
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