期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 295, 期 -, 页码 1-6出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.05.064
关键词
beta-glucuronidase; RhB@MOF-5; Inner filter effect; Metal organic framework
资金
- National Natural Science Foundation of China [21505084, 21775089, 21475074]
- Key Research and Development Program of Shandong Province [2017GSF19109]
- Innovation Project of Shandong Graduate Education [SDYY16091]
- Natural Science Foundation Projects of Shandong Province [ZR2014BM029]
beta-Glucuronidase (beta-GCU) is closely related to the occurrence of multiple diseases, and it has been applied as a biomarker and therapeutic target in clinical diagnosis. However, reliable methods with high selectivity and sensitivity for monitoring beta-GCU are still lacked. Herein, we designed a novel fluorescent nanoprobe, rhodamine B encapsulated MOF-5 (RhB@MOF-5) for the first time, to detect beta-GCU through the synergistic effect of inner filter effect (IFE) and static quenching effect (SQE) by employing 4-nitrophenyl-beta-D-glucuronide (PNPG) as the substrate. After encapsulating into MOF-5, the fluorescence emission of RhB at 550 nm excited by 320 nm was greatly enhanced. The major overlap between the fluorescence excitation spectrum of RhB@MOF-5 (about 320 nm) and the ultraviolet absorption spectrum of PNPG (about 310 nm) lead to PNPG being a good IFE absorber in this sensing system. Under the optimized conditions, the excitation spectrum of the RhB@MOF-5 could be absorbed by PNPG, resulting in the dramatically decrease of fluorescence emission. After adding beta-GCU into the system, the substrate of PNPG would be enzymatic hydrolyzed to p-nitrophenol (PNP) and glucose, then the IFE disappearance and the fluorescence recovered. The current sensing platform was interference-free and exhibited a broad linearity relationship for beta-GCU range of 0.1-10 U L-1 (R-2 = 0.9957) with a limit of detection as low as 0.03 U L-1, which was reduced by more than one orders compared with the reported methods. Moreover, the encapsulation of dyes using porous nanoparticles to achieve some tailor-made characteristics will enrich experimental design inspiration.
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