期刊
SCIENCE
卷 365, 期 6460, 页码 1469-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aax4804
关键词
-
资金
- NSERC
- NSF [1745301]
- National Institutes of Health [R01EB018975, U54CA199090]
- Heritage Medical Research Institute
- Packard Fellowship for Science and Engineering
- Burroughs Welcome Fund Career Award at the Scientitic Interface
The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene cluster derived from bacteria, we engineered a eukaryotic genetic program whose introduction into mammalian cells results in the expression of intracellular air-filled protein nanostructures called gas vesicles, which produce ultrasound contrast. Mammalian acoustic reporter genes allow cells to be visualized at volumetric densities below 0.5% and permit high-resolution imaging of gene expression in living animals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据