期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 116, 期 39, 页码 19743-19752出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1900317116
关键词
oligogalacturonides; plant-pathogen interaction; Arabidopsis thaliana; Botrytis cinerea; pectin lyase
资金
- Agence Nationale de la Recherche [ANR-12-BSV5-0001, ANR-14-CE34-0010-03]
- Laboratoire d'Excellence Saclay Plant Science project Dynano
- Institut Universitaire de France
- LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS]
- Agence Nationale de la Recherche (ANR) [ANR-14-CE34-0010] Funding Source: Agence Nationale de la Recherche (ANR)
Despite an ever-increasing interest for the use of pectin-derived oligogalacturonides (OGs) as biological control agents in agriculture, very little information exists-mainly for technical reasons-on the nature and activity of the OGs that accumulate during pathogen infection. Here we developed a sensitive OG profiling method, which revealed unsuspected features of the OGs generated during infection of Arabidopsis thaliana with the fungus Botrytis cinerea. Indeed, in contrast to previous reports, most OGs were acetyl- and methylesterified, and 80% of them were produced by fungal pectin lyases, not by polygalacturonases. Polygalacturonase products did not accumulate as larger size OGs but were converted into oxidized GalA dimers. Finally, the comparison of the OGs and transcriptomes of leaves infected with B. cinerea mutants with reduced pectinolytic activity but with decreased or increased virulence, respectively, identified candidate OG elicitors. In conclusion, OG analysis provides insights into the enzymatic arms race between plant and pathogen and facilitates the identification of defense elicitors.
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