期刊
PLOS ONE
卷 14, 期 9, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0217345
关键词
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资金
- Ministero della Salute [RF 2013-02356924]
- Associazione italiana per la ricerca sul cancro (AIRC 2013)
- Associazione italiana per la ricerca sul cancro (AIRC Fellowship 2017) [19499]
Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma(null) (NSG) mice engrafted with human cord blood (hCB)-CD34(+) cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34(+) cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34(+) NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34(+) NSG and controls suggests a poor human immune response against not compatible hAMLs. Our findings suggest that (hCB)-CD34(+) NSG mice are transient and/or incomplete carriers of the human immune system and, therefore, represent a suboptimal tool to study the interaction between tumor and immune cells.
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