期刊
PLANT JOURNAL
卷 101, 期 2, 页码 384-400出版社
WILEY
DOI: 10.1111/tpj.14549
关键词
RNA virus; potyvirus; Turnip mosaic virus; Arabidopsis thaliana; Nicotiana benthamiana; dynamin; adaptor protein 2; endocytosis; virus replication complex; host factor
资金
- National Natural Science Foundation of China [31701766, 31101411]
- Natural Science Foundation of Jiangsu Province [BK20161374]
- A-base grant from AAFC
- Natural Sciences and Engineering Research Council of Canada (NSERC)
Endocytosis and endosomal trafficking play essential roles in diverse biological processes including responses to pathogen attack. It is well established that animal viruses enter host cells through receptor-mediated endocytosis for infection. However, the role of endocytosis in plant virus infection still largely remains unknown. Plant dynamin-related proteins 1 (DRP1) and 2 (DRP2) are the large, multidomain GTPases that participate together in endocytosis. Recently, we have discovered that DRP2 is co-opted by Turnip mosaic virus (TuMV) for infection in plants. We report here that DRP1 is also required for TuMV infection. We show that overexpression of DRP1 from Arabidopsis thaliana (AtDRP1A) promotes TuMV infection, and AtDRP1A interacts with several viral proteins including VPg and cylindrical inclusion (CI), which are the essential components of the virus replication complex (VRC). AtDRP1A colocalizes with the VRC in TuMV-infected cells. Transient expression of a dominant negative (DN) mutant of DRP1A disrupts DRP1-dependent endocytosis and supresses TuMV replication. As adaptor protein (AP) complexes mediate cargo selection for endocytosis, we further investigated the requirement of AP in TuMV infection. Our data suggest that the medium unit of the AP2 complex (AP2 beta) is responsible for recognizing the viral proteins as cargoes for endocytosis, and knockout of AP2 beta impairs intracellular endosomal trafficking of VPg and CI and inhibits TuMV replication. Collectively, our results demonstrate that DRP1 and AP2 beta are two proviral host factors of TuMV and shed light into the involvement of endocytosis and endosomal trafficking in plant virus infection.
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