4.8 Article

A plant RNA virus hijacks endocytic proteins to establish its infection in plants

期刊

PLANT JOURNAL
卷 101, 期 2, 页码 384-400

出版社

WILEY
DOI: 10.1111/tpj.14549

关键词

RNA virus; potyvirus; Turnip mosaic virus; Arabidopsis thaliana; Nicotiana benthamiana; dynamin; adaptor protein 2; endocytosis; virus replication complex; host factor

资金

  1. National Natural Science Foundation of China [31701766, 31101411]
  2. Natural Science Foundation of Jiangsu Province [BK20161374]
  3. A-base grant from AAFC
  4. Natural Sciences and Engineering Research Council of Canada (NSERC)

向作者/读者索取更多资源

Endocytosis and endosomal trafficking play essential roles in diverse biological processes including responses to pathogen attack. It is well established that animal viruses enter host cells through receptor-mediated endocytosis for infection. However, the role of endocytosis in plant virus infection still largely remains unknown. Plant dynamin-related proteins 1 (DRP1) and 2 (DRP2) are the large, multidomain GTPases that participate together in endocytosis. Recently, we have discovered that DRP2 is co-opted by Turnip mosaic virus (TuMV) for infection in plants. We report here that DRP1 is also required for TuMV infection. We show that overexpression of DRP1 from Arabidopsis thaliana (AtDRP1A) promotes TuMV infection, and AtDRP1A interacts with several viral proteins including VPg and cylindrical inclusion (CI), which are the essential components of the virus replication complex (VRC). AtDRP1A colocalizes with the VRC in TuMV-infected cells. Transient expression of a dominant negative (DN) mutant of DRP1A disrupts DRP1-dependent endocytosis and supresses TuMV replication. As adaptor protein (AP) complexes mediate cargo selection for endocytosis, we further investigated the requirement of AP in TuMV infection. Our data suggest that the medium unit of the AP2 complex (AP2 beta) is responsible for recognizing the viral proteins as cargoes for endocytosis, and knockout of AP2 beta impairs intracellular endosomal trafficking of VPg and CI and inhibits TuMV replication. Collectively, our results demonstrate that DRP1 and AP2 beta are two proviral host factors of TuMV and shed light into the involvement of endocytosis and endosomal trafficking in plant virus infection.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据