4.7 Article

RNA Helicases from the DEA(D/H)-Box Family Contribute to Plant NMD Efficiency

期刊

PLANT AND CELL PHYSIOLOGY
卷 61, 期 1, 页码 144-157

出版社

OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcz186

关键词

DEA(D/H)-box RNA helicases; Nonsense-mediated mRNA decay; P-bodies; RNA quality control; UPF1

资金

  1. National Science Centre [UMO-2012/05/D/NZ1/00030, UMO2014/15/B/NZ2/02302, UMO2018/28/T/NZ1/00077, UMO-2015/19/N/NZ2/00200]
  2. EMBO Short-Term Fellowship [ASTF-396-2015, STF-7351]
  3. Agence Nationale de la Recherche [ANR-14-CE10-0015, ANR3'ModRN: ANR-15-CE12-0008-12]
  4. National Research, Development and Innovation Office [NKFIH K-129177, K-116963]
  5. European Union-the European Regional Development Fund Innovative economy 2007-13 [POIG.02.02.00-14-024/08-00S]

向作者/读者索取更多资源

Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic RNA surveillance mechanism that degrades aberrantm RNAs comprising a premature translation termination codon. The adenosine triphosphate (ATP)-dependent RNA helicase up-frameshift 1 (UPF1) is a major NMD factor in all studied organisms; however, the complexity of this mechanism has not been fully characterized in plants. To identify plant NMD factors, we analyzed UPF1-interacting proteins using tandem affinity purification coupled to mass spectrometry.Canonical members of the NMD pathway were found along with numerous NMD candidate factors, including conserved DEA(D/H)-box RNA helicase homologs of human DDX3, DDX5 and DDX6, translation initiation factors, ribosomal proteins and transport factors. Our functional studies revealed that depletion of DDX3 helicases enhances the accumulation of NMD target reporterm RNAs but does not result in increased protein levels. In contrast, silencing of DDX6 group leads to decreased accumulation of the NMD substrate. The inhibitory effect of DDX6-like helicases on NMD was confirmed by transient over-expression of RH12 helicase. These results indicate that DDX3 and DDX6 helicases in plants have a direct and opposing contribution to NMD and act as functional NMD factors.

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