期刊
PHYTOPATHOLOGY
卷 109, 期 12, 页码 2022-2032出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PHYTO-03-19-0103-R
关键词
bioinformatics; 'Candidatus Liberibacter asiaticus'; citrus limon; citrus sinensis; huanglongbing; metabolomics; microbiome; plant-microbe interactions; plant pathogen interactions; roots
资金
- California Citrus Research Board [5300-150]
- Ministry of Science and Technology, Taiwan, Republic of China [NSC-102-2911-I-005-301, NSC-103-2911-I-005-301]
- U.S. Department of Agriculture National Institute of Food and Agriculture Hatch Project [1005945]
- NIH [RR011973]
- NIFA [812383, 1005945] Funding Source: Federal RePORTER
Huanglongbing (HLB) is a severe, incurable citrus disease caused by the bacterium 'Candidatus Liberibacter asiaticus' (CLas). Although citrus leaves serve as the site of initial infection, CLas is known to migrate to and colonize the root system; however, little is known about the impact of CLas infection on root metabolism and resident microbial communities. Scions of 'Lisbon' lemon and 'Washington Navel' orange grafted onto 'Carrizo' rootstock were grafted with either CLas-infected citrus bud-wood or uninfected budwood. Roots were obtained from trees 46 weeks after grafting and analyzed via H-1 nuclear magnetic resonance spectroscopy to identify water-soluble root metabolites and high-throughput sequencing of 16S rRNA and ITS gene amplicons to determine the relative abundance of bacterial and fungal taxa in the root rhizosphere and endosphere. In both citrus varieties, 27 metabolites were identified, of which several were significantly different between CLas(+) and control plants. CLas infection also appeared to alter the microbial community structure near and inside the roots of citrus plants. Nonmetric multidimensional scaling (NMDS) and a principal coordinate analysis (PCoA) revealed distinct metabolite and microbial profiles, demonstrating that CLas impacts the root metabolome and microbiome in a manner that is variety-specific.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据