期刊
OSTEOARTHRITIS AND CARTILAGE
卷 27, 期 12, 页码 1831-1840出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2019.08.006
关键词
Mesenchymal stem/progenitor cells; Primary MSC; Synovium; Osteoarthritis; Chondrogenesis; MSC subpopulations; MSC isolation
资金
- Medical Research Council UK [MRC-MR-L022893]
- Dutch Arthritis Foundation [17-2-101, CO-14-1002]
- NWO [P15-23]
- Rush Klaus Kuettner Chair for Osteoarthritis Research
Objective: Synovium contains multipotent progenitor/stromal cells (MPCs) with potential to participate in cartilage repair. Understanding the identity of these MPCs will allow their therapeutic potential to be fully exploited. Hence this study aimed to identify primary synovial MPCs and characterize them in the context of cartilage regeneration. Methods: Primary MPC/MPC-subset specific markers in synovium were identified by FACS analysis of uncultured cells. MPC-subsets from human synovium obtained from patients undergoing total knee arthroplasty were FACS sorted, cultured, immunophenotyped and chondrogenically differentiated. The anatomical localization of MPCs in synovium was examined using immunohistochemistry. Finally, the presence of these MPC subsets in healthy synovium obtained from human organ donors was examined. Results: A combination of CD45, CD31, CD73 and CD90 can isolate two distinct MPC-subsets in synovium. These MPC-subsets, freshly isolated from synovium, did not express CD45 or CD31, but expressed CD73. Additionally, a sub-population of CD73(+) cells also expressed CD90. CD45(-)CD31(-)CD73(+)CD90(-) cells were significantly more chondrogenic than CD45(-)CD31(-)CD73(+)CD90(+) cells in the presence of TGFb1. Interestingly, reduced chondrogenic ability of CD73(+) CD90(+) cells could be reversed by the addition of BMP2, showing discrete chondrogenic factor requirements by distinct cell-subsets. In addition, these MPCs had distinct anatomical localization; CD73 was expressed both in intimal and sub-intimal region while CD90 was enriched in the sub-intimal region. We further demonstrated that these subsets are also present in healthy synovium. Conclusions: We provide indications that primary MPCs in synovial intima and sub-intima are pheno-typically and functionally distinct with different chondrogenic properties. (C) 2019 Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.
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