期刊
NUCLEIC ACIDS RESEARCH
卷 47, 期 18, 页码 9524-9541出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz660
关键词
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资金
- Spanish Ministry of Economy and Competitiveness
- European Union funds (FEDER) [BFU2013-48643-C3-1-P, BFU2016-77728-C3-1-P, BFU2013-48643-C3-3-P, BFU2016-77728-C3-3-P, BFU2015-71978-REDT, BFU2013-48643-C3-2-P, BFU2016-77728-C3-2-P]
- FPI, Spanish Government
- Regional Andalusian Government [P12-BIO1938MO, BIO271, BIO258]
- Regional Valencian Government [PROMETEO II 2015/006]
- Spanish Ministry of Economy and Competitiveness [BFU2016-77728-C3-1-P]
Co-transcriptional imprinting of mRNA by Rpb4 and Rpb7 subunits of RNA polymerase II (RNAPII) and by the Ccr4-Not complex conditions its post-transcriptional fate. In turn, mRNA degradation factors like Xrn1 are able to influence RNAPII-dependent transcription, making a feedback loop that contributes to mRNA homeostasis. In this work, we have used repressible yeast GAL genes to perform accurate measurements of transcription and mRNA degradation in a set of mutants. This genetic analysis uncovered a link from mRNA decay to transcription elongation. We combined this experimental approach with computational multi-agent modelling and tested different possibilities of Xrn1 and Ccr4 action in gene transcription. This double strategy brought us to conclude that both Xrn1-decaysome and Ccr4-Not regulate RNAPII elongation, and that they do it in parallel. We validated this conclusion measuring TFIIS genome-wide recruitment to elongating RNAPII. We found that xrn1 Delta and ccr4 Delta exhibited very different patterns of TFIIS versus RNAPII occupancy, which confirmed their distinct role in controlling transcription elongation. We also found that the relative influence of Xrn1 and Ccr4 is different in the genes encoding ribosomal proteins as compared to the rest of the genome.
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