Dietary nitrate supplementation alters the oral microbiome but does not improve the vascular responses to an acute nitrate dose

Nitrate (NO3-) contained in food and beverages can transiently increase nitric oxide (NO) availability following a stepwise reduction to nitrite (NO2-) by commensal bacteria in the oral cavity. We tested the hypothesis that regular ingestion of dietary NO3- would influence the oral microbiome, the capacity to reduce NO3- to NO2- in saliva, and the vascular responses to an acute dose of NO3-. The abundance of bacterial species on the tongue, the availability of NO markers, and vascular function were assessed in 11 healthy males before and after 7 days of supplementation with NO3--rich beetroot juice and a NO3--depleted placebo. As expected, saliva and plasma NO2- and NO3- were significantly elevated after NO3- supplementation (all P < 0.05) but not placebo. We found that NO3- supplementation increased salivary pH (7.13 +/- 0.54 to 7.39 +/- 0.68, P = 0.043) and altered the abundance of some bacteria previously implicated in NO3- reduction: Neisseria (from 2% +/- 3%-9% +/- 5%, P < 0.001), Prevotella (from 34% +/- 17%-23% +/- 11%, P = 0.001) and Actinomyces (from 1% +/- 1%-0.5% +/- 0.4%). Despite these alterations to the oral microbiota, an acute dose of NO3- increased salivary and plasma NO2-, reduced systolic blood pressure and increased the response to flow mediated dilation to a similar extent before and after 7 days of supplementation (P > 0.05). Our study establishes that supplementing the diet with NO3- for a sustained period can alter the oral environment in favour of health but does not impact the response to an acute NO3- dose. Acute ingestion of NO3- results in transient improvements in vascular function but the dietary induced adaptations to the oral bacteria did not enhance these effects.