期刊
NEW PHYTOLOGIST
卷 224, 期 2, 页码 848-859出版社
WILEY
DOI: 10.1111/nph.16138
关键词
alkaloid; Apocynaceae; Catharanthus roseus; idioblast cell; Imaging MS; laticifer cell; secondary metabolism; single-cell MS
资金
- MEXT KAKENHI [22120006]
- JSPS KAKENHI [14J03616, 24710235]
- Grants-in-Aid for Scientific Research [14J03616, 24710235] Funding Source: KAKEN
Catharanthus roseus is a medicinal plant well known for producing bioactive compounds such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). Although the leaves of this plant are the main source of these antitumour drugs, much remains unknown on how TIAs are biosynthesised from a central precursor, strictosidine, to various TIAs in planta. Here, we have succeeded in showing, for the first time in leaf tissue of C. roseus, cell-specific TIAs localisation and accumulation with 10 mu m spatial resolution Imaging mass spectrometry (Imaging MS) and live single-cell mass spectrometry (single-cell MS). These metabolomic studies revealed that most TIA precursors (iridoids) are localised in the epidermal cells, but major TIAs including serpentine and vindoline are localised instead in idioblast cells. Interestingly, the central TIA intermediate strictosidine also accumulates in both epidermal and idioblast cells of C. roseus. Moreover, we also found that vindoline accumulation increases in laticifer cells as the leaf expands. These discoveries highlight the complexity of intercellular localisation in plant specialised metabolism.
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