期刊
NEUROPHARMACOLOGY
卷 155, 期 -, 页码 54-64出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuropharm.2019.05.018
关键词
Spinal cord injury; Nanocurcumin; Inflammatory response; Neuroregeneration; Astrogliosis; Axonal growth
资金
- project InterAction from Operational Program Research, Development and Education in the framework of the project Center of Reconstructive Neuroscience [LTAUSA 17120, CZ.02.1.01/0.0./0.0/15_003/0000419]
- EATRIS-CZ [LM2015064]
- Ministry of Education, Youth and Sports of CR [LQ1604]
- European Regional Development Fund [CZ.2.16/3.1.00/21527]
- public budgets of the Czech Republic through the Operational Programme Prague - Competitiveness
- [CZ.1.05/1.1.00/02.0109]
A highly water soluble, nano-formulated curcumin was used for the treatment of the experimental model of spinal cord injury (SCI) in rats. Nanocurcumin and a vehicle nanocarrier as a control, were delivered both locally, immediately after the spinal cord injury, and intraperitoneally during the 4 consecutive weeks after SCI. The efficacy of the treatment was assessed using behavioral tests, which were performed during the experiment, weekly for 9 weeks. The behavioral tests (BBB, flat beam test, rotarod, motoRater) revealed a significant improvement in the nanocurcumin treated group, compared to the nanocarrier control. An immunohistochemical analysis of the spinal cord tissue was performed at the end of the experiment and this proved a significant preservation of the white matter tissue, a reduced area of glial scaring and a higher amount of newly sprouted axons in the nanocurcumin treated group. The expression of endogenous genes (Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, Nfk beta) and interleukins(IL-1 beta, TNF-alpha, IL-6, IL-12, CCL-5, IL-11, IL-10, IL-13) was evaluated by qPCR and showed changes in the expression of the inflammatory cytokines in the first two weeks after SCI.
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