期刊
NATURE METHODS
卷 16, 期 9, 页码 866-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-019-0512-x
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资金
- Academy of Finland (Center of Excellence project) [307415, 282192, 312491]
- Sigrid Juselius Foundation
- Finnish Medical Foundation
- Paulo Foundation
- Alfred Kordelin Foundation
- Maud Kuistila Foundation
- Biomedicum Helsinki Foundation
- Emil Aaltonen Foundation
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron minilAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.
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