期刊
NATURE CHEMICAL BIOLOGY
卷 15, 期 9, 页码 925-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41589-019-0340-4
关键词
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资金
- Cancer Center Support Grant [P30CA046934]
- School of Energy Resources at the University of Wyoming
- National Institutes of Health [2P20GM103432, R01GM118792]
Multicellular organisms achieve greater complexity through cell divisions that generate different cell types. We engineered a simple genetic circuit that induces asymmetric cell division and subsequent cell differentiation in Escherichia coli. The circuit involves a scaffolding protein, PopZ, that is stably maintained at a single cell pole over multiple asymmetric cell divisions. PopZ was functionalized to degrade the signaling molecule, c-di-GMP. By regulating synthesis of functionalized PopZ via small molecules or light, we can chemically or optogenetically control the relative abundance of two distinct cell types, characterized by either low or high c-di-GMP levels. Differences in c-di-GMP levels can be transformed into genetically programmable differences in protein complex assembly or gene expression, which in turn produce differential behavior or biosynthetic activities. This study shows emergence of complex biological phenomena from a simple genetic circuit and adds programmable bacterial cell differentiation to the genetic toolbox of synthetic biology and biotechnology.
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