期刊
NATURE BIOTECHNOLOGY
卷 37, 期 10, 页码 1145-+出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41587-019-0254-4
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资金
- IBS [IBS-R021-D1]
- National Research Foundation of Korea [2017R1D1A1B03035094, 2017R1E1A1A01074529, 2018M3A9H3021707, 2018M3A9H3022412]
- Next Generation BioGreen 21 Program [PJ01319301]
- Korea Healthcare Technology RD Project [HI16C1012]
- Technology Innovation Program [20000158]
- National Research Foundation of Korea [2018M3A9H3022412, 2017R1D1A1B03035094, 2018M3A9H3021707, 2017R1E1A1A01074529] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
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