A new antibacterial tirucallane from Walsura trichostemon roots

Phytochemical investigation of the roots of Walsura trichostemon, a Thai medicinal plant, provided a new tirucallane, 3-epimesendanin S 12-acetate (1), together with four known compounds, 3-epimesendanin S (2), meliasenin G (3), beta-sitosterol (4) and beta-sitosterol glucoside (5). Their structures were characterized by intensive spectroscopy including 1 D and 2 D NMR, UV, IR and MS spectrometric analysis. The isolated compounds were evaluated for antibacterial and acetylcholinesterase inhibitory activities. Compounds 1-2 showed antibacterial activity against Bacillus cereus and Bacillus subtilis with MIC values ranging from 16-128 mu g/mL. In addition, compound 3 was active against Pseudomonas aeruginosa and Escherichia coli with MIC values of 64 and 128 mu g/mL, respectively.

Automated summary

Phytochemical investigation of the roots of Walsura trichostemon, a Thai medicinal plant, led to the discovery of a new tirucallane and four known compounds. These compounds showed antibacterial and acetylcholinesterase inhibitory activities, with the new compound exhibiting promising results against certain bacteria.