期刊
MOLECULES
卷 24, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/molecules24173154
关键词
starfish; Patiria (=Asterina) pectinifera; polar steroids; colorectal carcinoma cells; X-ray; colony formation; apoptosis; DNA fragmentation
资金
- RFBR [18-34-20013]
Despite significant advances in the understanding, prevention, and treatment of cancer, the disease continues to affect millions of people worldwide. Chemoradiation therapy is a rational approach that has already proven beneficial for several malignancies. However, the existence of toxicity to normal tissue is a serious limitation of this treatment modality. The aim of the present study is to investigate the ability of polar steroids from starfish Patiria (=Asterina) pectinifera to enhance the efficacy of radiation therapy in colorectal carcinoma cells. The cytotoxic activity of polar steroids and X-ray radiation against DLD-1, HCT 116, and HT-29 cells was determined by an MTS assay. The effect of compounds, X-ray, and their combination on colony formation was studied using the soft agar method. The molecular mechanism of the radiosensitizing activity of asterosaponin P-1 was elucidated by western blotting and the DNA comet assay. Polar steroids inhibited colony formation in the tested cells, and to a greater extent in HT-29 cells. Asterosaponin P-1 enhanced the efficacy of radiation and, as a result, reduced the number and size of the colonies of colorectal cancer cells. The radiosensitizing activity of asterosaponin P-1 was realized by apoptosis induction through the regulation of anti-and pro-apoptotic protein expression followed by caspase activation and DNA degradation.
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