期刊
MOLECULES
卷 24, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/molecules24193461
关键词
ultrasound-assisted extraction (UAE); Angelica keiskei; flavonoids; antioxidant activity; response surface methodology (RSM)
资金
- China Postdoctoral Science Foundation [2016M602103]
- Shandong Provincial Natural Science Foundation [ZR2019PH020]
- Innovation-Driven Boost Project of Qingdao Science and Technology Association [C2018ZL]
Angelica keiskei Koidzumi (A. keiskei), as a Japanese edible herbal plant, enjoys a variety of biological activities due to the presence of numerous active compounds, especially flavonoids. This study aims for the optimization of ultrasound-assisted extraction (UAE) for flavonoids in A. keiskei and their antioxidant activity by using the response surface methodology (RSM). Single-factor experiments and a four-factor three-level Box-Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction and antioxidant activity evaluation: ultrasonic temperature (X-1), ultrasonic time (X-2), ethanol concentration (X-3) and liquid-solid ratio (X-4). The optimum conditions of the combination of total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity (DPPH-RSC) and ferric-reducing antioxidant power (FRAP) were as follows: X-1 = 80 degrees C, X-2 = 4 min, X-3 = 78%, X-4 = 35 mL/g, respectively. The experimental results provide a theoretical basis for the extensive utilization of A. keiskei and flavonoids extraction from A. keiskei as a potential source of antioxidants.
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