期刊
MOLECULAR MEDICINE REPORTS
卷 20, 期 4, 页码 3355-3362出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2019.10566
关键词
microRNA; cisplatin; cyclin D2; cell proliferation; lung cancer
资金
- National Natural Science Foundation of China [81772281, 31371321]
- Shandong Science and Technology Committee [ZR2019MH022, 2017GSF221011, 2018GSF118056]
- Yantai Science and Technology Committee [2016ws044, 2018XSCC 051]
- Health and Family Planning Commission of Shandong [2015WS0499]
- Shandong Province Taishan Scholar Project [ts201712067]
MicroRNAs (miRNAs/miRs) serve important roles in the chemotherapeutic effect of anticancer drugs. To investigate the roles of miRNAs in cisplatin-induced suppression of lung adenocarcinoma cell proliferation, A549 cells were treated with different concentrations of cisplatin. An MTT assay demonstrated that cisplatin inhibited A549 cell proliferation in a dose-dependent manner. Cisplatin induced cell apoptosis and inhibited cell migration by increasing the levels of miR-93, miR-26a and miR-26b. Furthermore, as an upstream factor, miR-93 was proposed to regulate cyclin D2 expression in miR-93-transfected A549 cells. Cisplatin also induced Bcl-2-associated X protein expression, and decreased that of Bcl-2 and c-Myc in lung adenocarcinoma cells. In vivo analysis further supported that cisplatin inhibited lung adenocarcinoma cell growth by regulating cyclin D2 and miR-93 expression. In conclusion, our findings demonstrated that cisplatin could effectively inhibit lung adenocarcinoma cell proliferation by decreasing cyclin D2 expression via miR-93.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据