期刊
MICROCHIMICA ACTA
卷 186, 期 8, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-019-3612-5
关键词
Lead ion; Electrochemical; Biosensor; DNAzyme; Hairpin-shaped substrate probe; Triple signal amplification; Catalytic hairpin assembly; 3D DNA-AuNP network; RuHex; Real water samples
资金
- National Natural Science Foundation of China [31471644]
- Primary Research & Development Plan of Shandong Province [2017GSF220009]
- Program for Taishan Scholer of Shandong Province [TS201712048]
- University of Jinan Scientific Research Fund [XKY1421]
- Doctoral Fundation of University of Jinan [XBS1431]
An amplified electrochemical biosensing scheme is described for lead(II) ions. It is making use of DNAzyme-assisted target recycling and catalytic hairpin assembly (CHA). The hairpin strand (substrate probe for the Pb2+-based DNAzyme; referred to as SP) is composed of trigger probe (TP) and a capture probe 1 attached to gold nanoparticles (AuNP). In the presence of the enzyme probe that partially hybridizes with SP, the introduction of Pb2+ triggers target recycling and drives the highly amplified translation of target Pb(II) to TP. The CHA reaction is further initiated by TP. The modified AuNP act as the connecting unit, and this leads to the formation of a 3D DNA-AuNP network on the electrode (which is the third amplification step). It can bind the positively charged redox mediator RuHex via electrostatic interaction for electrochemical detection. This biosensor has a low detection limit (95 pM) and any analytical range that covers the 100 pM to 5 mu M Pb(II) concentration range. It is selective over other divalent metal ions. It was applied to the determination of Pb2+ in spiked real-world samples.
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